A comparative study of VITEK-2, Double Disc Synergy and Combined Disc Methods for detection of ESBL (Extended Spectrum Beta-Lactamase) production in Escherichia coli and Klebsiella pneumoniae strains
نویسندگان
چکیده
Aim: In this prospective study we aimed to compare the effectiveness of VITEK-2 (bioMérieux, France) automated system, double-disc synergy (DDST) versus combined disc test (CDT) in detecting Extended-Spectrum Beta-Lactamase (ESBL) positivity Escherichia coli and Klebsiella pneumoniae strains isolated from various clinical samples. Material Method: E.coli K. inoculated on Mueller Hinton Agar plate. Susceptibility tests were performed with VITEK 2 (BioMérieux, system before. Afterward, EBSL was investigated manually DDST CDT. Minimal inhibitor concentration (MIC) results three compared each other according European Committee Antimicrobial Testing (EUCAST) criteria. Results: 184 E. K.pneumoniae strains, elevated. 92.9% 98 patients positive DDS method, 100% 86 negative MDC. Conclusion: found have a sensitivity 100%, specificity 92.4%, predictive value 92.8% 100%. be compatible validation for ESBL positivity.
منابع مشابه
Evaluation of Extended Spectrum Betalactamase(ESBL) Positive Strains of Klebsiella pneumoniae And Escherichia coli in Bacterial Cultures
Background and Objective: To evaluate extended spectrum betalactamase (ESBL) positive strains of Klebsiella pneumonia and Escherichia coli in positive bacterial cultures. Materials and Methods: In this analytical cross-sectional study, between March 2006 and March 2007, 170 bacterial isolates including 133 cases of E. coli and 37cases of K. pneumonia were...
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1. Chambers HF. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microb Rev 1997; 10: 781–91. 2. Brown DFJ. Detection of methicillin/oxacillin resistance in staphylococci. J Antimicrob Chemother 2001; 48 Suppl S1: 65–70. 3. Skov R, Smyth R, Larsen AR et al. Evaluation of cefoxitin 5 and 10 mg discs for the detection of methicillin resistan...
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BACKGROUND AND OBJECTIVES Various phenotypic methods are recommended in the routine practice to detect the ESBL production in gram negative bacilli. Among them, the Double Disc Synergy Test (DDST) which uses the third generation cephalosporins (3GC), is a simple and a reliable method. But the coexistence of AmpC may give false negative results. In such cases, the ESBL detection can be improved ...
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ژورنال
عنوان ژورنال: Journal of contemporary medicine
سال: 2022
ISSN: ['2146-6009', '2146-4189']
DOI: https://doi.org/10.16899/jcm.954056